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7 However, mast cells can be activated to perform important effector and immunomodulatory functions by mechanisms that are independent of IgE

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Rush AJ, Trivedi MH, Wisniewski SR, Nierenberg AA, Stewart JW, Warden D, et al

Quantitative real-time PCR (qRT-PCR) analysis Total RNA was isolated from cortical samples by using RNAiso plus (Takara, Shiga, Japan) according to the manufacturers instructions