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Anti-TNF agents and ustekinumab appear to be effective for both inducing and maintaining fistula closure, although only infliximab has been assessed in randomized trials with fistula closure as the primary end point

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Patients who stop at this point typically retain results for 4 to 8 months before drifting back to their untreated trajectory

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Insoluble immune complexes were rinsed once with Co-IP buffer then eluted by 0.1 mM citrate (pH3.0) twice for 5 min, resolved by sodium dodecyl sulfatepolyacrylamide gel, and transferred to nitrocellulose