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A total of 1.2 g of RNA was utilized for reverse transcription, accomplished using the MMLV Reverse Transcriptase kit (M1701, Promega, Walldorf, Germany), Random Hexamer Primers (48,190,011, Thermo Fisher), and RiboLock RNase Inhibitor (EO0384, Thermo Fisher)

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Hydroxyl Radicals in E-Cigarette Vapor and E-Vapor Oxidative Potentials under Different Vaping Patterns