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Intracellular reactive oxygen species production may cause cellular damage ( Reference Streck, Vieira and Wannmacher 9 ) and disorders of the antioxidant defence system ( Reference Blundell, Jones and Rose 12 ) , demonstrating a mechanistic connection between hyperhomocysteinaemia, oxidative stress and disease
3 multiple-use glass vials 30mL each
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FTIR spectroscopy A Bruker Vertex FTIR-micro spectrometer was used for the analysis of the samples
The homogenizer was stopped and the solution was placed on a magnetic stirring plate, covered with parafilm and mixed with a magnetic stir bar until cooled to room temperature
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