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For BrdU staining, the primary BrdU antibody was added together with 0.5 U l 1 DNAse (Sigma, D5025) and 1 mM MgCl 2 (Sigma, M8266) in the blocking solution and incubated for 30 min at room temperature

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Liquid chromatographymass spectrometry (LC-MS/MS) analysis of this protein lysate identified LRP1 as an ECM1-interacting protein (Additional file 6, Fig

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