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Clean the area with an alcohol wipe

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Cell viability was assessed using a Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) according to the manufacturers instructions, with absorbance measured at 450 nm

GF status was confirmed through 16S qPCR analysis before use in relevant experiments, which were also carried out at Shenzhen Gnotobio Biotechnology Co., Ltd

Goldstein, I

These findings provide a comprehensive structural framework for developing next-generation anti-obesity drugs based on dual receptor activation mechanisms