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Description

After 24 h, viability was assessed using the AquaBluer assay as previously described ( Determination of GSH Levels HT-1080 cells were cultured in DMEM containing 10% FBS, 1% supplemented non-essential amino acids and 1% Pen-Strep mixture (all from Gibco), and maintained in a humidified atmosphere with 5% CO 2 at 37C

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The redox energy supply of nicotinamide adenine dinucleotide (NAD + ) is vital to glycolysis and the tricarboxylic acid cycle (TCA), forming free NADH (reduced form) in the mitochondria

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