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After completion of the transblotting process, the membranes were washed three times with TBS buffer pH 7.3 (8 g NaCl, 0.2 g KCl, and 3 g Tris-base/ 1 liter) for 10 minutes

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doi: 10.1016/j.bbagen.2012.11.016

The key is to stay hydrated with water and avoid excessive caffeine and alcohol consumption