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Cells were plated on 48-well plates, coated with anti-CD3 (1 g ml 1 ) and anti-CD28 (10 g ml ) antibodies, in RPMI medium or DMEM, supplemented with 10% FCS, penicillin, streptomycin, HEPES, glutamine, pyruvate, nonessential amino acids and -mercaptoethanol diluted with PBS (1:1), in the presence of IL-2 (10 ng ml 1 , Peprotech) and IL-12 (10 ng ml 1 , Peprotech) (T H 1 conditions), with or without amino acids

B.et al (2007)

A child has a higher proportion of total body water, a smaller circulating blood volume, and kidneys that are still maturing, all of which make them more vulnerable to the rapid fluid shifts that an IV infusion can trigger

This study involved only non-invasive application of a test hydrogel on the dorsal hand skin of a healthy adult volunteer for photographic documentation

Livdans-Forret AB, Harvey PJ, Larkin-Thier SM