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Determination of yeast cytosolic pH was performed as previously described ( ADH plasmid containing a pH-sensitive ratiometric GFP variant named pHluorin (kindly obtained from S

Cell viability and cell-free tyrosinase inhibition assay Considering the potential of selected flavonoids as mh-Tyr inhibitors and so as an active ingredient for the formulation against hyperpigmentation, evaluation of these compounds for their cell viability efficacy in mammalian cell lines is required before furthering the experimental analysis

clinically, somatropin is used to correct growth-hormone deficiency [7] [8]

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Lipids were extracted using the Folch procedure, and phosphorus was determined by a micromethod as described previously (7, 21)

GPx-1 prevents the accumulation of hydrogen peroxide and thereby protects against oxidative stress