In addition, deletion of the GSH1 geneencoding -glutamylcysteine synthetase (-GCS), the enzyme catalysing the first step in glutathione synthesiswas shown to suppress an Ero1 conditional mutant, again indicating that GSH provides an additional load on Ero1 activity (Frand & Kaiser, 1998)
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The main phenotype of loss-of-function mutations in arGSTs is the decreased content of anthocyanins ( 1 ) 19,20,21,22,23,24,25,26,27,28 and proanthocyanidins 27 , which can be well explained by an incomplete biosynthesis due to the absence of an essential enzyme
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For a person weighing 150 pounds (roughly 68 kg), this would mean a daily intake of 1,360 mg to 2,720 mg
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