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Likewise, in dose and application regimens as described before heparin (Belupo, Croatia), warfarin (Martefarin (Orion Pharma, Finland)), L-NAME (Sigma, USA) and L-arginine (Sigma, USA) dissolved in saline were used as well [1,14,15]

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Primary antibodies used for detecting shelterin subunits were custom rabbit anti-TRF1 (1:5,000, Thermo Scientific), mouse anti-TRF2 antibody (1:10,000, Abcam ab13579) and mouse anti-TIN2 antibody (1:10,000, Novus Biologicals 59B388.1)