In vitro culture increases the frequency of stochastic epigenetic errors at imprinted genes in placental tissues from mouse concepti produced through assisted reproductive technologies
Additionally, the upregulation of inflammation-related genes, such as IL-6 and TNF-, is associated with the pathological state of depression, suggesting that immune responses play a critical role in its pathogenesis [365, 366]
Warsinah 2017)
doi: 10.5230/jgc.2015.15.1.10 7 HooiJKYLaiWYNgWKSuenMMYUnderwoodFETanyingohDet al
11shows immunohistochemistry images of explant cultures stained with Neurofilament (green), when incubated under different treatment (and time) conditions showing different number of surviving spiral ganglion neurons
Prostate cancer and melanoma experiments in mice were conducted in the BIOS+ animal facility in Bellinzona under specific pathogen-free conditions, with approval from project licences 35293, 36369 and 37480