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After incubation on ice for 15 min, 25 l of 10% (v/v) Nonidet P-40 (Nacalai Tesque, Kyoto, Japan) was added to the cell suspension, followed by vortexing for 10 s at maximum speed

These microplastics were collected immediately after use and before release into the environment

The streets will be much cleaner if smoking is banned

They are the first combustible cigarettes to receive this designation

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After decades away from his most iconic character, Reubens reprised the role for Broadways Pee-wee Herman Show in 2010, then cowrote and starred in the 2016 Netflix film Pee-wees Big Holiday