Irie et al
Careful methods protect patients from preventable healthcare infections
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Protein O-GlcNAcylation: emerging mechanisms and functions
DNMT activity assay In accordance with the manufacturers instructions, we extracted nuclear proteins with an Active Motif Nuclear Extraction Kit (Active Motif, Carlsbad, USA) and evaluated DNMT activity with an EpiQui DNA Methyltransferase Activity/Inhibition Assay Kit (Epigentek, Farmingdale, USA) as reported previously 64
In nonbiological samples, Craig and Bartlett previously observed biphasic decay of MeHg in the presence of H 2 S in an aqueous reaction mixture, with an initial rapid decay and then a slow decay that were probably due to the formation of (MeHg) 2 S, which is a somewhat water-insoluble material, and evolution of DMeHg from the reaction mixture, respectively 27 , thus supporting the results of our cell-free study