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Transmission electron microscopy and BODIPY staining Samples collected from each infection stage were fixed in 2.5% ( v/v ) glutaraldehyde at 4 C overnight, and were washed with 0.1 M phosphate buffer saline (PBS), post-fixed in 1% ( w/v ) osmic acid for 2 h, washed again with 0.1 M PBS, dehydrated sequentially with ethanol gradient, and infiltrated with epoxy

Track these metrics for several weeks to assess response accurately

Tested, every batch

Treadmill exhaustion test As described 64 , mice were acclimated to the treadmill (Columbus Instruments, Exer-6M Open Treadmill) 23 days before the test

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Why is bacteriostatic water used with peptides