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These alterations in the tumor ECM induce an increase in its stiffness, typically evaluated using techniques that either require direct contact with the sample (e.g., atomic force microscopy), or measure the tumor mechanical properties in a contact-free manner (e.g., shear wave elastography, magnetic resonance elastography) (Table 3)

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Swim latency, swim distance, time spent in the target quadrant, and number of quadrant crossings were recorded using a computerized video tracking system [1]