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For negative-mode acquisition, mobile phase A was 100% LCMS-grade H 2 O with 10 mM ammonium acetate, 0.1% ammonium hydroxide and 0.1% medronic acid (Agilent)

Several studies have found that ferroptosis is involved in the regulation of the hepatic microenvironment and various hepatic metabolisms, thereby mediating the progression of related liver diseases

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