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doi: 10.1038/cr.2011.22

Reduction and alkylation of the cysteine residues were then performed with 10 mM DTT and 55 mM iodoacetamide, respectively, followed by adding 360 L of 50 mM ammonium bicarbonate buffer to reduce the urea concentration to below 1 M

Comparison of the inhibitory effects of resveratrol and tranilast on IgE, 48/80 and substance P dependent-mast cell activation