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For instance, GSH, composed of cysteine, glycine, and glutamic acid, accounts for the majority of cellular antioxidants to create highly reductive TME and is involved in tumor initiation, progression, and metastasis (Harris et al., 2015

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For viability, 7-aminoactinomycin D (7AAD) (Thermo fisher A1310) or Zombie NIR (BioLegend 423106) diluted 1:3000 was stained concurrently with antibodies in all flow cytometry assays

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