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We therefore assessed the neutralization capacity of the two kinds of MNPs (RBC-NPs and MDCK-NPs) to recombinant ETX with Glutathione-S-transferase (GST) tags (GST-ETX) in vitro by 3-(4,5-Dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt (MTS) assay with cells

Abstract Ergothioneine (ET) is an unusual sulfur-containing derivative of the amino acid, histidine, which is derived exclusively through the diet

The protein concentration was quantified using a BCA kit, and then SDS-PAGE buffer was added

A buildup of toxins in the cells causes the unnatural darkening of epidermal tone

Scientists have noted that the simplicity of the molecule gives consistent results across different experimental conditions and is stable under standard lab storage conditions

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