2022 and earlier Zagreb dossiers)
ROS interaction with these macromolecules can cause lipid peroxidation, gene mutation, enzyme inactivation and protein denaturation (Nathan and Cunningham-Bussel, 2013
Methods Materials GSH, GSSG, 2-mercaptoethanol, ophthalmic acid, S -methylglutathione and yeast glutathione reductase (GR) were obtained from Sigma-Aldrich, HEDS was from Alfa Aesar, GSSCys from Toronto Research Chemicals and NADPH was from Gerbu
On the next day, the medium was replaced with fresh DMEM (which contains 5 M cystine) containing [ 14 C] cystine (0.04 Ci, PerkinElmer) with/without 1 M erastin, and cells were incubated for the indicated time periods
These methods were described in Albrecht et al
Treatment Sessions and Recovery Most treatments for skin brightening are quick, often lasting under an hour