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In another set of experiments, ASA treatments (5 or 10 mol/ml ASA 48 h) were performed in GSH-depleted cells (BSO treatment for 18 h) which were then treated with 10 mM NAC (dissolved in DMSO, then diluted with cell culture medium to the required concentration keeping the DMSO concentration not more than 0.1%) for 2 hr before the addition of ASA for 48 h to manipulate cellular GSH pools

In: Calcium: The Molecular Basis of Calcium Action in Biology and Medicine

We thank Mark Cleasby, PhD, from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript

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Avoid presenting symptom improvement as proof of tissue regeneration