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Consistently, Reactome (REAC) analysis revealed that CHL-upregulated proteins were significantly enriched in the innate immune system ( p adjusted = 3.1E-12), complement cascade ( p adjusted = 6.6E-07) and antimicrobial peptides ( p adjusted = 3.5E-08)

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Total RNA was extracted and reverse-transcribed into cDNA using the RNAsimple Total RNA Kit and the PrimeScript RT Reagent Kit with gDNA Eraser