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Free glutathione, GSH, is read first after 15 minutes, followed by addition of a reaction mixture that converts all the oxidized glutathione, GSSG, into free GSH, which then reacts with the excess detection reagent to yield the signal related to Total GSH content

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Kornblau SM , Ruvolo P, Y-H Q, Patel S, Thomas D, Giles FJ, Beran M, Estrov Z, May WS, Andreeff M

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