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Cells resuspended in the growth medium (GM, DMEM containing 10% fetal bovine serum (FBS, Life Technologies) and 1% anti-anti), and the single cells were seeded in a cell culture flask

Possible process of compromised immunotolerance in women is asymmetrical X chromosome inactivation (XCI), which cause evade from the presentation of X-linked self-antigens in the thymus and following deterioration of T cell tolerance

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Methods: To elucidate this, we examined the effects of an NO scavenger (cPTIO), and an exogenous NO donor (GSNO), on broccolini postharvest yellowing