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These discrepancies might be partially based on differences in sampling, as Vishwakarma and Yadav have employed a larger sample size than Ferrario et al

Increasing O-GlcNAcylation is neuroprotective in young and aged brains after ischemic stroke

In this case, the aliquot of the samples were immediately mixed with 0.5 ml of 1% zinc acetate to capture the maximum amount of sulfide in the biological material and incubated at 37.5C for 10 min