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Immunoprecipitation and immunoblotting Extraction of proteins from cultured cells was performed with a modified lysis buffer (50 mM Tris-HCl [pH 7.5], 0.1% SDS, 1% Triton X-100, 150 mM NaCl, 1 mM dithiothreitol, 0.5 mM EDTA, 100 M PMSF, 100 M leupeptin)

Amer HM, Wahba HE, Marrez DA, Salama AB, Khalid KA

A study examining GHK's effects on human breast cancer MCF7 and prostate cancer PC3 cell lines found gene expression changes consistent with reduced proliferative and invasive capacity

Zhou, N, Vlahos, I, Hooda, Z, Hofstetter, WL, Mehran, RJ, Rajaram, R, Rice, DC, Sepesi, B, Swisher, SG , Vaporciyan, AA, Walsh, GL, Mitchell, KG, Antonoff, MB

Suppl.,2006, 5(4), 418-429 9

Many now expect both testing methods to validate SPF claims