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After being washed in PBS, the slides were stained with hematoxylin and eosin, mounted with Canada balsam (03984, Sigma-Aldrich), and observed with an epifluorescence microscope (IX71, Olympus, Tokyo, Japan) equipped with a digital imaging system (DP71, Olympus)

The cells were cultured in a cell incubator at 37 C, 5% CO2, and 95% humidity

User 15 cited a general dosing range of 2.5 to 3.75mcg per kilogram of body weight twice daily, which, at higher body weights, still produces doses within the standard 250 to 500mcg range being used across the dataset

This is what we've learned from years of supplying these compounds to top-tier research institutions

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Store the injection below 30C as advised on the label, and keep it away from heat, sunlight, and children