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& Simon, M

Verification of Target Engagement: Measure 1-MNA levels (should decrease) Measure NAD+ levels (should increase) Both confirm on-target NNMT inhibition 3

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& Mullin, G

Consistent with the distribution of Firmicutes in the cecal samples, the relative abundance of Ileibacterium was highest in the WD controls (average relative abundance 68%), second highest in the LD controls (average relative abundance 51%), and lowest in the vehicle- and 5A-1MQ-treated mice switched from WD to LD (average relative abundances of 40% and 41%, respectively)

Reproducibility benchmarks: Because AOD-9604 has been extensively characterized, it is frequently used as a positive control in new lipolysis assay development