doi: 10.1007/s11239-022-02642-4
Selenium: biochemical role as a component of glutathione peroxidase
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It is capable of binding protein in both native and denaturing conditions.The use of denaturing condition of becomes important when proteins are found in inclusion bodies and must be denatured so they can be solubilized
Glucose/galactose adapted cells were cultured in DMEM containing 10% dFBS, 100 U ml 1 penicillin and 100 g ml 1 streptomycin, 1 mM sodium pyruvate and 25 mM glucose (Sigma-Aldrich, G7021-1KG) or 25 mM galactose (Sigma-Aldrich, G5388-500G) for a minimum of 1 month before being used experimentally