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Avoid the inner buttocks completely because of the sciatic nerve risk

doi: 10.1016/j.toxlet.2005.10.018

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Follicle health and quantification To assess ovarian reserves, ovaries were collected from diestrus stage animals, embedded in resin, subject to thick (20 m) sections for the entire ovary, with every third section digitally scanned for stereology analysis, and follicle reserve and health manually counted on a grid using Stereo Investigator software

The homogenates were sonicated (20 min), followed by centrifugation at 10,000 rpm (15 min at 20 C) to collect the supernatant