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Referenced Citations Khavinson, V.K., D.M
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The supernatant was discarded and the beads were washed thrice with LSB-2 containing 150 mM NaCl which is termed LSB-3 (10 mM Tris pH 7.4, 1 mM EDTA, 10 mM -mercaptoethanol, 150 mM NaCl, and 1 mM PMSF) with or without IGEPAL CA-630 followed by incubation at 4 C/1 h