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Results High levels of caffeine in donor red blood cells are reproducible across time To assess the extent and physiological impact of caffeine exposure on stored RBC quality, we first quantified caffeine levels in packed RBC units from 13,091 donors enrolled in the REDS RBC Omics study

After being boosted through precursor supplementation, NAD+ often plateaus and declines around week 12, which is likely due to an overabundance of NAM (a byproduct of NAD+) and a shortage of methyl donors present to fuel conversion in the salvage pathway.38 Supplementing with a potent methyl donor can offset this shortage and ensure sustained activity in the salvage pathway