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Western blot analysis and Coomassie Brilliant Blue (CBB) staining Samples were boiled in sample loading buffer at 100 C for 10 min, separated by SDS-PAGE, and transferred onto NC membranes for blotting

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Unverified purity: Grey-market IGF-1 DES is a fragile protein easily degraded or mislabeled

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This gives it different absorption properties and oral bioavailability , making it more accessible for non-injectable protocols

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