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The animal studies were approved by the Animal Experimental Ethical Committee of Fudan University (number: 2019 Huashan Hospital JS-112) and performed in accordance with the guidelines for the care and use of laboratory animals set by Fudan University (Shanghai, China)

Then the samples from the three cities were combined, and a multinomial logistic regression model was used to examine the association between smoking and drinking and binge drinking, controlling for age, gender, race/ethnicity, education, and city