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We also solved the crystallographic structures of the C408S, C201S/C408S, and C201S CstB mutants to 2.48 , 2.48 and 3.11 resolution, respectively, by molecular replacement with the wild-type enzyme (see Table S1 for crystallographic statistics)

All transient transfections of plasmids into cell lines followed the standard protocol for Polyethylenimine Linear (PEI) Transfection Reagent (24765-1, Polyysciences Inc)

High-pH RP fractionation was performed at a flow rate of 0.6 mL/min with a gradient starting at 5% B for 2 min, increased to 50% B in 12 min, to 70% B within 0.5 min, and held for 6.5 min

It was found that subjects receiving PUVA and PL had a higher proportion of patients with repigmentation of 50% or more [137]

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