Briefly, 100 l of fresh mitochondria extracted from liver tissues were mixed with 900 l staining working solution and then the fluorescence intensity was assessed using a fluorescence microplate reader (BioTek, Winooski, VT, USA)
If any changes phisically, discard product
Changes of isometric tension were measured with force transducer (FORT 10, WPI, Sarasota, FL, USA)
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