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The GSH chromatography ligand is immobilized to the stationary phase by the central cysteine sulfhydryl group through a linker but maintains the same net electrostatic charge as with intracellular GSH at physiological pH (2 positive and 1 negative) (Tummanapelli and Vasudevan, 2015)

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doi: 10.7150/thno.53506 16 NguyenTTTShangEWesthoffM-AKarpel-MasslerGSiegelinMD