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Tissue was homogenized in 1 ml of FACS and centrifuged at 1000 RPM for 5 min, followed by removing the supernatant and adding 1 ml of FACS buffer, centrifuged at 1000 RPM for 5 min, repeated it three times

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Lutein significantly enhanced basic endothelial function as presented by amplified NO, declined endothelin (ET-1) release, and attenuated inflammatory mediators release [58]

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