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M.AmorimM

It promotes the formation of new collagen and elastin while regulating enzymes (MMPs) that break down excess or poorly formed scar tissue, replacing it with stronger, healthier structures

The remaining supernatant was mixed with 10 mM N-ethylmaleimide, rotated at 4 C for 1 h and precleared with Pierce Protein G magnetic beads (Thermo Fisher Scientific, 88848) by rotating at 4 C for 1 h

Initially, one injection intramuscularly per week on or around the same day for a maximum of 6 weeks

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An omnivorous diet allows for the B12 intake through animal products (with a bioavailability ranging from 24% to 36% in eggs, up to 65% in lean meat) (Doets et al., 2013), while vitamin B12 is scarce or absent in diets including little or no animal products