Another important factor with retractions is that they appear to be more common in journals with higher impact factors (Brembs, Button & Munaf, 2013), and this should not surprise us as these journals are prone to publishing studies with confirmation bias (Forstmeier, Wagenmakers & Parker, 2017
Research suggests starting with lower concentrations and increasing gradually, monitoring for any signs of irritation
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After mixing, it is placed at 4 for 10 minutes