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The effect of melatonin on anxiety and pain of tourniquet in intravenous regional anesthesia

4.3.2 SIRT1/Nfr2/HO-1/GPX4 The activity of Nrf2 signaling is enhanced by SIRT1 via increasing its expression, decreasing the expression of Keap1, and promoting its binding ability to antioxidant response element (ARE) (the promoter region of downstream antioxidant enzymes) (Patel et al., 2022)