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Wilmer et al

Then, 100 L mixed internal standard solution and 400 L methanol solution (precooled at -20C) were added to each sample and vortexed thoroughly for 60 s

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at five to six months, from 0% to 7%

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Because FOXO4-DRIs mechanism depends on disrupting the FOXO4p53 interaction, and because this interaction is only operative in cells with elevated FOXO4 expression, the compound preferentially targets the senescent cell population leaving FOXO4-low non-senescent cells largely unaffected