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Subcluster analysis has revealed both differences within cell types in healthy and disease tissues suggesting that analysis of single cell data at higher resolution can give insight into both normal physiological functions as well as pathophysiological responses

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Quantitative Real Time-PCR analysis Using the technique of real-time PCR to detect changes in AEC iNOS expression with 1% CSS, a progressive fall in copy number was seen over 24 h (Figure 5)

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