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The replacement of the media is essential for removing the CuCl 2 and Cu(OAc) 2 interference before assaying the LDH levels as they are shown to reduce the fluorescence signal read-out within treated wells

Second, light

2021 doi: 10.1093/plphys/kiab159

Troubleshooting Reference Chart When protocols do not work as expected, systematic troubleshooting helps identify the issue

10.1124/dmd.111.040469 Drug Metab