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You could have therapeutic levels of tirzepatide circulating in your blood during the draw, and the results would say nothing about it

Allosteric opening of the polypeptide-binding site when an Hsp70 binds ATP

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Acknowledgements The authors would like to express their gratitude to Margreet Osinga (Gelderse Vallei Hospital, Ede, the Netherlands), Annelies Botman (Spaarne Gasthuis, Haarlem, the Netherlands), Fellery de Lange (Medical Center Leeuwarden, Leeuwarden, the Netherlands), Jantine van Holten (Ikazia Hospital, Rotterdam, the Netherlands), Jankees K.H

After treatment, cells were fixed with 4% paraformaldehyde (PFA) for 15 min before permeabilization with 0.1% saponin in blocking buffer (1% fatty acid free BSA, 20 mM glycine in PBS) for 15 min at room temperature