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Inhibition of Glutamate cysteine ligase depletes neuronal glutathione, elevates glutamate and increases excitatory neurotransmission Glutamate cysteine ligase (GCL) is the rate limiting step for glutathione synthesis, and utilizes glutamate as a substrate

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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GMCSF

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