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Nevertheless, longer-term exposures are frequently studied, often in combination with multiple time points, to examine the time-course variation of the metabolome, to understand the sequence of molecular initiating events leading to an adverse outcome pathway, and chronic toxicity upon repeated exposure to a given compound ( 2.3 Importance of including control and negative reference compounds A key aspect in drug-toxicity evaluation by in vitro metabolomics is the importance of including data from both non-hepatotoxic compounds as well as non-treated cells ( in vitro metabolomics studies, for instance, are often overlooked but can be a significant source of bias in the data, sometimes even more intense than the effects of the drug being tested

1992;29(1):6568 Lenzi A, Picardo M, Gandini L, Dondero F