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All constructs were cloned into a pcDNA3.1(+) vector backbone (ThermoFisher Scientific) and confirmed by sequencing analysis

Some individuals experience a sensation of warmth, flushing, or slight dizziness, especially if the infusion or injection is given rapidly

Further analysis confirmed that FOXO4-positive cells co-expressed Leydig cell markers, specifically StAR and CYP11A1, indicating that FOXO4 is indeed expressed in human Leydig cells [1]